The 15th European Microscopy Congress
Manchester Central, United Kingdom
was held on 16th -
© Royal Microscopical Society 2012
emc2012 was organised by
the Royal Microscopical
Society
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LS1.4: Imaging brain structure and function across different spatial and temporal scales
Session co-organisers: Angus Silver, David DiGregorio, Zoltan Nusser
Brain function is poorly understood. A key reason for this is that it is difficult to study the underlying mechanisms because they occur across a wide range of spatial and temporal scales. Here we focus on the development and application new imaging methods for studying synaptic neuronal and network function. This 2.5 day symposium will bring together leading experts, who will present their latest studies covering areas including the molecular properties of synapses (synaptomics), dendritic integration in neurons, in vivo functional imaging of neural populations and large scale connectivity (connectomics).
Contributions covering the applications of microscopy in this field, as well as developments in instrumentation and techniques, are strongly encouraged.
Wednesday 19th September AM
Synaptic structure function
10:00 Diverse distribution of voltage-gated ion channels on the surface of hippocampal CA1 pyramidal cells
Zoltan Nusser (Invited) Hungarian Academy of Sciences, Hungary
10:30 Estimates of vesicle diffusion and active-zone supply rates at cerebellar mossy fibre terminals using FRAP and 3D reaction-diffusion simulations
Jason Rothman (Invited) University College London, UK
11:00 Break
11:15 Targeted presynaptic patch-clamp using Scanning Ion Conductance Microscopy
U.Vivekananda (Contributed) Department of Clinical and Experimental Epilepsy, Institute of Neurology, London, UK
11:30 Dual-color superresolution imaging of synapses and glia cells in living brain slices using STED microscopy
Valentin Nägerl (Invited) University of Bordeaux, France
U.Vivekananda (Contributed) Department of Clinical and Experimental Epilepsy, Institute of Neurology, London, UK
11:30 Dual-
Valentin Nägerl (Invited) University of Bordeaux, France
Wednesday 19th September PM
Synaptic structure function
14:00 SINGLE VESICLE/MOLECULE STUDIES OF CALCIUM TRIGGERED SYNAPTIC VESICLE FUSION
Axel T Brunger (Invited) Stanford University, USA
14:30 Imaging nanoscale mobility in cellular space using time-resolved fluorescent anisotropy imaging microscopy
K.Zheng (Contributed) UCL, Institute of Neurology, London, UK
14:45 3D-visualisation of input synapses of a motion-detecting neuron using scanning electron microscopy
Gerd Leitinger (Contributed) Medical University of Graz, Graz, Austria
Gerd Leitinger (Contributed) Medical University of Graz, Graz, Austria
15:00 Break
15:15 Serial Section Scanning Electron Microscopy (S3EM) on Silicon Wafers for Ultrastructural Volume Imaging of Cells and Tissues
C.Koerber (Contributed) Institute of Anatomy and Cell Biology, Heidelberg University, Heidelburg, Germany
15:30 Deep Synapse Diversity: Why it Matters and How to Measure it
Stephen Smith (Invited) Stanford University, USA
Neuronal function
10:00 Dendritic computations by thin dendrites of cerebellar interneurons
David DiGregorio (Invited) Pasteur Institute, France
10:30 Properties of dendrites of pyramidal neurons in anterior cingulate cortex
Thomas Nevian (Invited) University of Bern, Switzerland
11:00 Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning
C.Ventalon (Contributed) University Paris Descartes, Neurophysiology and New Microscopies Laboratory, CNRS UMR 8154, Inserm U603, Paris, France
C.Ventalon (Contributed) University Paris Descartes, Neurophysiology and New Microscopies Laboratory, CNRS UMR 8154, Inserm U603, Paris, France
11:15 Investigation of dendritic integration in spiny stellate cells of barrel cortex with 2-photon uncaging
E.Chaigneau (Contributed) University College London, Department of Neuroscience, London, UK
11:30 Compartmentalization and cooperation of synaptic inputs
E.Chaigneau (Contributed) University College London, Department of Neuroscience, London, UK
11:30 Compartmentalization and cooperation of synaptic inputs
Judit Makara (Invited) Hungarian Academy of Sciences, Hungary
Neuronal function and Networks: structure to function
14:00 Exploring the mouse visual cortex in vivo with two-photon calcium imaging
Nathalie Rochefort (Invited) Institute of Neuroscience and CIPS, Technische Universität München, Germany
14:30 Activation and suppression of specific neuronal subpopulations enables efficient encoding during natural movies
MF.Iacaruso (Contributed) University College London, London, UK
14:45 Genetically encoded miniSOG probes for light and EM imaging: applications to neural tracing and cell biological analysis in Drosophila
J.Ng (Contributed) MRC-LMB, Cambridge, UK
15:00 Break
15:30 Structural Neurobiology: The dense reconstruction of neuronal circuits
Moritz Helmsteadter (Invited) Max Planck Institute of Neurobiology, Germany
Friday 21st September AM
Networks: structure to function
10:00 Towards an understanding of the intrinsic biophyisical diversity and connectivity of mammalian neurons
Troy Margrie (Invited) MRC National Institute for Medical Research, UK
Troy Margrie (Invited) MRC National Institute for Medical Research, UK
10:30 Imaging Cortical Vasculature with Stimulated Raman Scattering and Two-Photon Photothermal Lensing Microscopy
J.Moger (Contributed) University of Exeter, UK
10:45 Fast hemodynamic responses in the awake visual cortex
Pisauro (Contributed) UCL Institute of Ophtalmology, University College London
11:00 Investigating the connectivity and function of neuronal networks in visual cortex with two-photon imaging
Thomas Mrsic-Flogel (Invited) University College London, UK
Thomas Mrsic-
11:30 A 2-photon acousto-optic lens (AOL) microscope for high-speed 3D imaging of neural activity
Angus Silver (Invited) University College London, UK
Angus Silver (Invited) University College London, UK
This session is sponsored by Coherent, Inc