The 15th European Microscopy Congress

Manchester Central, United Kingdom

was held on 16th - 21st September 2012

The European Microscopy Society presents

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LS2.1: Super-resolution fluorescence microscopy for life sciences
Session co-organisers: Mark Bates, Michelle Peckham
Fluorescence microscopy, a powerful tool for the study of cell and molecular biology, has undergone a period of rapid development in recent years.  New concepts for optical imaging with nanometer-scale spatial resolution have led to new opportunities for the observation of biological ultrastructure.  In general, these methods rely on a number of photophysical or photochemical mechanisms by which fluorescent probes may be converted between a detectable "on" state and a non-detectable "off" state, either at spatially well-defined regions of the sample (e.g. the STED method) or in a stochastic manner through the detection of photo-switchable single molecules (e.g. the PALM/STORM method).  This session will highlight recent technical advances in super-resolution fluorescence microscopy, and will also focus on applications of these methods to biological imaging.
Monday 16th September PM
14:00 Principles of Optical Nanoscopy

Andreas Schoenle (Invited) GWDG, Germany

14:30 Photo-switchable fluorophores for super-resolution fluorescence microscopy

Mark Bates (Invited) Max Planck Institute, Germany

15:00 Dual colour photoactivation localisation microscopy of flotillin microdomains

N.Barry (Contributed) MRC Laboratory of Molecular Biology, Cambridge, UK
15:15 Nanometric particle localization and tracking in three dimensional cellular biology

Yang (P.Dalgarno) (Contributed) Heriot Watt University, Edinburgh, UK

15:30 Reproducible super-resolution microscopy

Alex Knight (Contributed) National Physical Laboratory, Teddington, UK

15:45 Algorithm Specific Comparison of Gen II sCMOS and EM-CCD Cameras for Precision Localization Microscopy using a Camera Simulation Engine

E Barker (Contributed) Systems Division, Hamamatsu Photonics UK Ltd, Welwyn Garden City, UK
Tuesday 17st September AM
10:00 Probing intracellular transport with correlated live cell and super resolution microscopy

Melike Lakadamyali (Invited) The Institute of Photonic Sciences, Spain
10:30 Multidimensional localization microscopy using conventional fluorochromes and single wavelength illumination

Mark Cannell (Invited) University of Bristol, UK
11:00 Using 3D PALM to image the Z-disc in cardiomyocytes

Y.Takagi (Contributed) NHLBI, Bethesda, USA
11:15 Structured illumination microscopy: A useful tool to analyse autofluorescent material within age-related sub-retinal pigment epithelium deposits in the human eye

Sabrina Rossberger (Contributed)Dep. of Ophthalmology, University Hospital Heidelberg, Germany

11:30 Test Samples for STORM Super-Resolution Imaging

D.Metcalf (Contributed) National Physical Laboratory, Teddington, UK

11:45 Microscopic Analysis of the Interaction of Corynebacterium diphtheriae with Mouse Macrophages revealed by Quantum Dot Sub Diffraction Imaging

R.Palmisano (Contributed) Friedrich-Alexander University, Germany

This session is sponsored by Hamamatsu