LS2.3: Probes for light and electron microscopy
Session co-organisers: Paul Verkade, Carsten Schultz
The availability of Green Fluorescent Protein (GFP) to specifically mark proteins has revolutionised life science light microscopy. Likewise gold particles have been an invaluable tool for localization studies in electron microscopy for decades. Probes are therefore a crucial part of almost any microscopy technology. Improvements to existing probes and the development of new ones give us even greater opportunities. In this session we will highlight the current state of the art of what is possible with these new and upcoming and well-established probes for both light and electron microscopy.
Thursday 20th September AM
10:00 Metallothionein and A3 peptide as clonable high-density markers for cryo-electron microscopy
Andreas Hoenger (Invited) University of Colorado, USA
10:30 A fluorescence electron microscope: cathodoluminescence detection gives the resolution of electron microscopy with colour discrimination
I Morrison (Contributed) University of York, York, UK
10:45 Labeling of ultrathin resin sections for correlative light and electron microscopy
Thomas Kurth (Contributed) U Dresden, CRTD, Dresden, Germany
11:00 New labels for multiple immunolabeling in high resolution SEM
Jana Nebesarova (Contributed) Biology Centre ASCR, Ceske Budejovice, Czech Republic
11:15 Improving detection of low abundant proteins and RNAs in plant tissues
A Timmers (Contributed) Laboratory of Plant Microorganism Interactions, Castanet-Tolosan, France
11:30 "New approaches for correlated LM and 3D EM applied to MULTISCALE CHALLENGES: Bridging Gaps in Knowledge and Understanding"
Mark Ellisman (Invited) National Centre for Microscopy and Imaging Research, USA