The 15th European Microscopy Congress
Manchester Central, United Kingdom
was held on 16th -
© Royal Microscopical Society 2012
emc2012 was organised by
the Royal Microscopical
Society
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LS2.4: 3D Image Processing (3D microscopy, 3D image analysis and developmental imaging)
Session co-organisers: Roland Fleck, Jiri Janacek
Introduction: 2 sessions will tackle the challenges of image processing in dynamic (living) and 3D systems. The new insight into systems biology offered by 3D visualisation of the building blocks of life are key to advancing our understanding of how living systems work. These visualisation challenges are at the basic structural units which assemble to form organelle through to the functional interactions between complex tissues in whole organisms. Microscopy plays a critical role in these studies, however, the processing of the images/ data generated is increasingly important in understanding these systems.
Session 1 will consider electron microscopy and 3D image analysis of spatial structures.
Session 2 will consider optical imaging solutions which offer news insight into complex multicellular systems.
10:00 Structure of macromolecular complexes in action by 4D single-particle cryo-EM
Marin van Heel (Invited) Imperial College London, UK
10:30 Modelling of image formation in cryo electron microscopy
M Vulovic (Contributed) Quantitative Imaging Group, Dept. of Imaging Science and Technology, Delft University of Technology, Delft, The Netherlands
10:45 Fast, spatially varying CTF correction in TEM
L.M. Voortman (Contributed) Delft University of Technology, Delft, The Netherlands
11:00 Imaging and tracking dynamic processes in live-cell microscopy
M Vulovic (Contributed) Quantitative Imaging Group, Dept. of Imaging Science and Technology, Delft University of Technology, Delft, The Netherlands
10:45 Fast, spatially varying CTF correction in TEM
L.M. Voortman (Contributed) Delft University of Technology, Delft, The Netherlands
11:00 Imaging and tracking dynamic processes in live-
Alan Greenaway (Invited) Heriot Watt University, UK
11:30 3D imaging using transmission-through-dye microscopy
Michael Model (Contributed) Kent State University, Kent, Ohio, USA
11:45 Simultaneous Compositional- and/or Topographical Material Analysis using Low Drift and High Speed Four Video-Outputs Backscattered Electron Detector (AsB4)
Michael Model (Contributed) Kent State University, Kent, Ohio, USA
11:45 Simultaneous Compositional-
Michel Aliman (Contributed) Carl Zeiss NTS GmbH, Oberkochen, Germany
Tuesday 18th September AM
Rainer Heintzmann (Invited) Jena University, Germany
10:30 OpenSPIM: An open access Selective Plane Illumination Microscope for the observation of developing fruitfly and zebrafish embryos
P Pitrone (Contributed) MPI-CBG, Dresden, Germany
P Pitrone (Contributed) MPI-
10:45 Quality control of 3D cell image analysis in optical microscopy
Michal Kozubek (Invited) Masaryk University, Czech Republic
11:15 Reductive evolution of large ribosomal subunits - High resolution electron microscopic ribosome reconstructions obtained with automated image acquisition on a direct detection device
Daniel Boehringer (Contributed) ETH Zurich
11:30 Morphometric SEM 3D analysis of microvascular networks and automated calculation of vessel angioarchitecture optimalities
Bernd Minnich (Contributed) University of Salzburg
11:45 Focused Ion Beam Ablation Tomography
C Parmenter (Contributed) The University of Nottingham