The 15th European Microscopy Congress

Manchester Central, United Kingdom

was held on 16th - 21st September 2012

The European Microscopy Society presents

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LS2.7: Correlative Microscopy for Life Sciences
Session co-organisers: Lucy Collinson, Peter O’Toole
Correlative Light and Electron Microscopy is a powerful technique that combines the benefits of fluorescence microscopy (large field-of-view, protein localisation) with the benefits of electron microscopy (nanometre resolution, ultrastructural context for markers). This session covers recent developments in technology that are pushing the boundaries of correlative imaging. Light microscopes are being integrated into electron microscopes to give seamless multimodal imaging with high correlation efficiency. Cryo light microscopy is being integrated with cryo electron microscopy for direct detection of expressed fluorescent markers with minimal processing artefacts and ice contamination. Finally, correlative imaging is extending to other imaging media, with correlative cryo fluorescence and cryo soft X-ray tomography delivering high resolution protein localisation and ultrastructural imaging of whole, unstained cells.

Wednesday 19th September PM
14:00 Zooming in on cellular architecture with cryo-correlative electron microscopy
Bram Koster (Invited) Leiden University Medical Center (LUMC) The Netherlands
14:30 iLEM, an integrated solution for correlative light and electron microscopy

Alevtyna Yakushevska (Contributed) FEI Company, Eindhoven, The Netherlands

14:45 Simultaneous light and electron microscopy

N Liv (Contributed) Delft University of Technology, Delft, The Netherlands

15:00 Correlative Light and Electron Microscopy of Neuronal Membrane Trafficking

C L Dennison (Contributed) University of York, York, UK

15:15 Biological Imaging at Diamond: The Cryo- Transmission Soft X-ray Microscopy Beamline

E Duke (Contributed) Diamond Light Source, Didcot, Oxfordshire, UK

15:30 Correlative cryofluorescence and soft X-ray tomography of autophagy in whole, unstained mammalian cells

L Collinson (Contributed) Cancer Research UK London Research Institute, London, UK

15:45 Analysis of Vaccinia virus infection by cryo-X-ray nano-tomography

Francisco García (Contributed) Centro Nacional de Biotecnología (CNB-CSIC, Madrid, Spain

Correlative cryofluorescence and soft X-ray tomography of autophagy in whole, unstained mammalian cells