The 15th European Microscopy Congress
Manchester Central, United Kingdom
was held on 16th -
© Royal Microscopical Society 2012
emc2012 was organised by
the Royal Microscopical
Specimen preparation is particularly important when imaging biological samples in the confocal and electron microscopes. Ideally, perfectly prepared immunofluorescence experiments should have a high signal to noise ratio and include a range of control samples. These parameters are often dictated by the quality of antibody, but the experts on the RMS Learning Zone may be able to offer hints and tips that could improve your sample preparation. Considerations in preparation such as choice of fixative and quality of antibody are important and the experts are happy to pass on their experiences and tips.
Standard chemical fixation of biological material for the electron microscope can induce numerous artefacts, however making slight changes (based on knowledge of the chemical interactions within your sample) could improve the quality of your sample preparation.
Microscopists from different backgrounds will be available to answer your questions
about specimen preparation, for EM and confocal including preparation of cells, tissues